Funded in part by Shiseido Co., Ltd.
Magnesium Supplementation Attenuates Ultraviolet-B-Induced Damage Mediated through Elevation of Polyamine Production in Human HaCaT Keratinocytes
Shu S, Kobayashi M, Marunaka K, Yoshino Y, Goto M, Katsuta Y, Ikari A
Cells · 4 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Industry funded
- Government
- Japan Society for the Promotion of Science
- Company
- Shiseido Co., Ltd.
- Grants
- Japan Society for the Promotion of Science (19H03373)
Based on 2 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2022-07-22 · Cells · vol. 11 · issue 15 · p. 2268
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 9 citations · more than 63% of similar papers · 0.6× the field average
- References
- 42 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Polyamine Metabolism and Applications · Skin Protection and Aging · Biomedical Research and Pathophysiology
- Keywords
- Polyamine, HaCaT, CREB, Downregulation and upregulation, GSK-3, Chemistry, Oxidative stress, Biochemistry, Protein kinase A, GSK3B, Cell biology, Kinase, Molecular biology, Biology, Transcription factor, In vitro
- MeSH
- cell line, keratinocytes, humans, magnesium, polyamines, glycogen synthase kinase 3, mitogen-activated protein kinase kinases, ultraviolet rays, dietary supplements, cyclic amp response element-binding protein
7 authors
From JP
- Shokoku ShuGifu Pharmaceutical University
- Mao KobayashiGifu Pharmaceutical University
- Kana MarunakaGifu Pharmaceutical University
- Yuta YoshinoGifu Pharmaceutical University
- Makiko GotoMirai Hospital
- Yuji KatsutaMirai Hospital
Abstract
Magnesium ions (Mg2+) have favorable effects such as the improvement of barrier function and the reduction of inflammation reaction in inflammatory skin diseases. However, its mechanisms have not been fully understood. Microarray analysis has shown that the gene expressions of polyamine synthases are upregulated by MgCl2 supplementation in human HaCaT keratinocytes. Here, we investigated the mechanism and function of polyamine production. The mRNA and protein levels of polyamine synthases were dose-dependently increased by MgCl2 supplementation, which were inhibited by U0126, a MEK inhibitor; CHIR-99021, a glycogen synthase kinase-3 (GSK3) inhibitor; and Naphthol AS-E, a cyclic AMP-response-element-binding protein (CREB) inhibitor. Similarly, reporter activities of polyamine synthases were suppressed by these inhibitors, suggesting that MEK, GSK3, and CREB are involved in the transcriptional regulation of polyamine synthases. Cell viability was reduced by ultraviolet B (UVB) exposure, which was rescued by MgCl2 supplementation. The UVB-induced elevation of reactive oxygen species was attenuated by MgCl2 supplementation, which was inhibited by cysteamine, a polyamine synthase inhibitor. Our data indicate that the expression levels of polyamine synthases are upregulated by MgCl2 supplementation mediated through the activation of the MEK/GSK3/CREB pathway. MgCl2 supplementation may be useful in reducing the UVB-induced oxidative stress in the skin.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
Community trust
Loading…
How much do you trust this study's findings?
Comments
Sign in to rate, comment on or flag this study.Sign inSomething wrong here?
Flag this study if its information, labels or funding look wrong. An editor reviews every flag.
Sign in to rate, comment on or flag this study.Sign inEducational information about published research. Not medical advice, and not a recommendation to start or stop anything.