15 N-labeled dietary nitrate supplementation increases human skeletal muscle nitrate concentration and improves muscle torque production
Kadach S, Park JW, Stoyanov Z, Black MI, Vanhatalo A, Burnley M, Walter PJ, Cai H, Schechter AN, Piknova B, Jones AM
Acta physiologica (Oxford, England) · 29 citations
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Health markers and function
- Intake measured by
- Not stated
Who paid for it
- Funding
- Independent funding
- University or hospital
- University of Exeter
- University or hospital
- University of Queensland
- Government
- Biotechnology and Biological Sciences Research Council
- Grants
- Biotechnology and Biological Sciences Research Council (BB/S020632/1); Biotechnology and Biological Sciences Research Council (BB/S020632/1)
Based on 3 listed funder(s).
Publication
- Published
- 2023-01-06 · Acta Physiol (Oxf) · vol. 237 · issue 3 · p. e13924
- Publisher
- Wiley
- Cited
- 35 citations · more than 99% of similar papers · 11.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 57 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Cardiovascular and exercise physiology · Nitric Oxide and Endothelin Effects · Pharmacological Effects and Assays
- Keywords
- Chemistry, Skeletal muscle, Internal medicine, Endocrinology, Crossover study, Ingestion, Nitric oxide, Bioavailability, Placebo, Animal science, Biochemistry, Medicine, Biology, Pharmacology
- MeSH
- muscle, skeletal, humans, nitrates, nitrites, nitric oxide, nitrogen dioxide, polyesters, cross-over studies, double-blind method, blood pressure, torque, dietary supplements
11 authors
From GB, US
- Stefan KadachUniversity of Exeter
- Ji Won ParkNational Institutes of Health; National Institute of Diabetes and Digestive and Kidney Diseases
- Zdravko StoyanovUniversity of Exeter
- Matthew I. BlackUniversity of Exeter
- Anni VanhataloUniversity of Exeter
- Mark BurnleyLoughborough University
Abstract
Aim
Dietary nitrate (NO3 - ) supplementation increases nitric oxide bioavailability and can enhance exercise performance. We investigated the distribution and metabolic fate of ingested NO3 - at rest and during exercise with a focus on skeletal muscle.
Methods
In a randomized, crossover study, 10 healthy volunteers consumed 12.8 mmol 15 N-labeled potassium nitrate (K15 NO3 ; NIT) or potassium chloride placebo (PLA). Muscle biopsies were taken at baseline, at 1- and 3-h post-supplement ingestion, and immediately following the completion of 60 maximal intermittent contractions of the knee extensors. Muscle, plasma, saliva, and urine samples were analyzed using chemiluminescence to determine absolute [NO3 - ] and [NO2 - ], and by mass spectrometry to determine the proportion of NO3 - and NO2 - that was 15 N-labeled.
Results
Neither muscle [NO3 - ] nor [NO2 - ] were altered by PLA. Following NIT, muscle [NO3 - ] (but not [NO2 - ]) was elevated at 1-h (from ~35 to 147 nmol/g, p 15 N-labeled. There was a significant reduction in 15 N-labeled muscle [NO3 - ] from pre- to post-exercise. Relative to PLA, mean muscle torque production was ~7% greater during the first 18 contractions following NIT. This improvement in torque was correlated with the pre-exercise 15 N-labeled muscle [NO3 - ] and the magnitude of decline in 15 N-labeled muscle [NO3 - ] during exercise (r = 0.66 and r = 0.62, respectively; p < 0.01).
Conclusion
This study shows, for the first time, that skeletal muscle rapidly takes up dietary NO3 - , the elevated muscle [NO3 - ] following NO3 - ingestion declines during exercise, and muscle NO3 - dynamics are associated with enhanced torque production during maximal intermittent muscle contractions.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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