The Extraction and High Antiproliferative Effect of Anthocyanin from Gardenblue Blueberry
Zhao F, Wang J, Wang W, Lyu L, Wu W, Li W
Molecules (Basel, Switzerland) · 16 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Jiangsu Agriculture Research System
- Government
- the 'JBGS' Project of Seed Industry Revitalization in Jiangsu Province
- Government
- the Jiangsu Province Key Project of R&D Plan (Modern Agriculture)
- Government
- Jiangsu Province Key Project of R&D Plan (Modern Agriculture)
- Government
- Jiangsu Agricultural Industry Technology System
- Grants
- Jiangsu Agriculture Research System (JATS [2022]510); Jiangsu Agriculture Research System (BE2020344)
Based on 5 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2023-03-22 · Molecules · vol. 28 · issue 6 · p. 2850
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 33 citations · more than 93% of similar papers · 2.8× the field average
- Impact
- Top 10% most cited in its field
- References
- 41 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Antioxidant Activities · Ginkgo biloba and Cashew Applications · Pomegranate: compositions and health benefits
- Keywords
- Anthocyanin, Apoptosis, Chemistry, HeLa, In vivo, Cancer cell, In vitro, Cell growth, Cisplatin, MTT assay, IC50, Biochemistry, Pharmacology, Cancer, Food science, Biology, Chemotherapy, Biotechnology
- MeSH
- humans, fruit, flavonoids, anthocyanins, plant extracts, antioxidants, cell proliferation, blueberry plants
6 authors
From CN
- Fengyi ZhaoInstitute of Botany; Institute of Botany, Jiangsu Province and Chinese Academy of Sciences; Jiangsu Provincial Key Laboratory for the Research and Utilization of Plant Resources
- Jialuan WangInstitute of Botany; Institute of Botany, Jiangsu Province and Chinese Academy of Sciences; Jiangsu Provincial Key Laboratory for the Research and Utilization of Plant Resources
- Weifan WangNanjing Forestry University
- Lianfei LyuInstitute of Botany; Institute of Botany, Jiangsu Province and Chinese Academy of Sciences; Jiangsu Provincial Key Laboratory for the Research and Utilization of Plant Resources
- Wenlong Wu · correspondingInstitute of Botany; Institute of Botany, Jiangsu Province and Chinese Academy of Sciences; Jiangsu Provincial Key Laboratory for the Research and Utilization of Plant Resources
- Weilin Li · correspondingNanjing Forestry University
Abstract
Blueberries are rich in flavonoids, anthocyanins, phenolic acids, and other bioactive substances. Anthocyanins are important functional components in blueberries. We collected 65 varieties of blueberries to investigate their nutritional and functional values. Among them, Gardenblue had the highest anthocyanin content, with 2.59 mg/g in fresh fruit. After ultrasound-assisted solvent extraction and macroporous resin absorption, the content was increased to 459.81 mg/g in the dried powder. Biological experiments showed that Gardenblue anthocyanins (L1) had antiproliferative effect on cervical cancer cells (Hela, 51.98 μg/mL), liver cancer cells (HepG2, 23.57 μg/mL), breast cancer cells (MCF-7, 113.39 μg/mL), and lung cancer cells (A549, 76.10 μg/mL), and no apparent toxic effects were indicated by methyl thiazolyl tetrazolium (MTT) assay, especially against HepG2 cells both in vitro and in vivo. After combining it with DDP (cisplatin) and DOX (doxorubicin), the antiproliferative effects were enhanced, especially when combined with DOX against HepG2 cells; the IC50 value was 0.02 μg/mL. This was further evidence that L1 could inhibit cell proliferation by inducing apoptosis. The detailed mechanism might be L1 interacting with DNA in an intercalation mode that changes or destroys DNA, causing apoptosis and inhibiting cell proliferation. The findings of this study suggest that L1 extract can be used as a functional agent against hepatoma carcinoma cells.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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