Physiological and biochemical responses to caffeine and microplastics in Mytilus galloprovincialis
Impellitteri F, Yunko K, Martyniuk V, Matskiv T, Lechachenko S, Khoma V, Mudra A, Piccione G, Stoliar O, Faggio C
The Science of the total environment · 46 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Università degli Studi di Messina
Based on 1 listed funder(s).
Publication
- Published
- 2023-05-23 · Sci Total Environ · vol. 890 · p. 164075
- Publisher
- Elsevier BV
- Cited
- 70 citations · more than 95% of similar papers · 3.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 135 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Microplastics and Plastic Pollution · Environmental Toxicology and Ecotoxicology · Marine Biology and Environmental Chemistry
- Keywords
- Mytilus, Microplastics, Oxidative stress, Viability assay, Catalase, Metallothionein, Superoxide dismutase, Glutathione, Lipid peroxidation, Chemistry, Biochemistry, Saliva, Biology, Environmental chemistry, Cell, Ecology, Enzyme
- MeSH
- animals, humans, caffeine, plastics, glutathione, glutathione disulfide, water pollutants, chemical, oxidative stress, mytilus, caspase 3, biomarkers, microplastics
10 authors
From IT, UA
- Federica ImpellitteriUniversity of Messina
- Kateryna YunkoTernopil Volodymyr Hnatiuk National Pedagogical University
- Viktoria MartyniukTernopil Volodymyr Hnatiuk National Pedagogical University
- Tetiana MatskivTernopil Volodymyr Hnatiuk National Pedagogical University; I.Horbachevsky Ternopil National Medical University
- Sofia A. LechachenkoTernopil Volodymyr Hnatiuk National Pedagogical University
- Vira KhomaTernopil Volodymyr Hnatiuk National Pedagogical University
Abstract
Caffeine (Caff) is one of the most widely used substances in the human diet and a well-recognized drug. Its input into surface waters is remarkable, but biological effects on aquatic organisms are unclear, particularly in combination with pollutants of suspected modulatory activity, like microplastics (MP). The aim of this study was to reveal the impact on the marine mussel Mytilus galloprovincialis (Lamark, 1819) of Caff (20.0 μg L-1) in the environmentally relevant combination (Mix) with MP 1 mg L-1 (size 35-50 μm) after the exposure for 14 days. Untreated and exposed to Caff and MP separately groups were also examined. Cell viability and cell volume regulation in hemocytes and digestive cells, as well as the indexes of oxidative stress, glutathione (GSH/GSSG) and metallothioneins levels, and caspase-3 activity in digestive gland were assessed. MP and Mix reduced Mn-superoxide dismutase, catalase, and glutathione S-transferase activities and level of lipid peroxidation, but increased the digestive gland cell viability, GSH/GSSG ratio (by 1.4-1.5-fold), metallothioneins level and their Zn content, while Caff did not affect oxidative stress indexes and metallothionein-related Zn chelation. Protein carbonyls were not targeted in all exposures. The distinguishing feature of the Caff group was the decline (2-fold) in caspase-3 activity and low cell viability. The multi-stress effect of Mix was shown by the worsening of the volume regulation of digestive cells and confirmed by discriminant analysis of biochemical indexes. The special capabilities of M. galloprovincialis as a sentinel organism make it an excellent bio-indicator reflecting the multi-stress effects in sub-chronic exposures to potentially harmful substances. The identification of the modulation of individual effects in combined exposure increases the need to base monitoring programs on studies of multi-stress effects in sub-chronic exposures.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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