Broccoli-Derived Exosome-like Nanoparticles Alleviate Loperamide-Induced Constipation, in Correlation with Regulation on Gut Microbiota and Tryptophan Metabolism
Duan T, Wang X, Dong X, Wang C, Wang L, Yang X, Li T
Journal of agricultural and food chemistry · 49 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Possibly industry funded
- Government
- National Natural Science Foundation of China
- Industry group
- Young Talent Fund of Association for Science and Technology in Shaanxi, China
- Government
- Youth Star of Science and Technology in Shaanxi Province
- University or hospital
- National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China
- Grants
- National Natural Science Foundation of China (32272309)
Based on 4 listed funder(s).
Publication
- Published
- 2023-10-24 · J Agric Food Chem · vol. 71 · issue 44 · pp. 16568–16580
- Publisher
- American Chemical Society
- Cited
- 65 citations · more than 99% of similar papers · 12.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 42 works
- Access
- Paywalled
- Research areas
- Gastrointestinal motility and disorders · Gut microbiota and health · Congenital gastrointestinal and neural anomalies
- Keywords
- Gut flora, Tryptophan, Feces, Constipation, Biology, Pharmacology, Biochemistry, Chemistry, Microbiology, Internal medicine, Medicine
- MeSH
- animals, humans, mice, brassica, constipation, loperamide, tryptophan, rna, ribosomal, 16s, neurotransmitter agents, nanoparticles, exosomes, gastrointestinal microbiome
7 authors
From CN
- Tianchi DuanShaanxi Normal University
- Xiaoyuan WangShaanxi Normal University
- Xinyue DongShaanxi Normal University
- Chennan WangShaanxi Normal University
- Lu WangShaanxi Normal University
- Xingbin YangShaanxi Normal University
Abstract
Constipation, a common gastrointestinal dysfunction, damages patients' life quality and predisposes them to other serious diseases. Current strategies against constipation often cause drug dependency and side effects. Here, we demonstrated that broccoli-derived exosome-like nanoparticles (BENs), a natural product with high gastrointestinal stability, ameliorated LOP-induced constipation in mice. Specifically, orally administered BENs (17.5 mg/kg/d) effectively shortened defecation time, sped up intestinal propulsion rate, and increased feces amount in constipated mice. BENs also raised excitatory neurotransmitters SP and MTL and reduced inhibitory neurotransmitters VIP and ET-1. Mechanistically, BENs were taken up by gut microbes, restored LOP-disordered gut microbiota, and altered microbial metabolism of SCFAs and tryptophan, as evidenced by the results of fluorescence microscopy, 16S rRNA gene sequencing, and nontargeted metabolomics. Thereinto, BEN-enriched SCFA-producing microorganisms are closely associated with the feces amount and SP and VIP levels and BEN-elevated indole-3-pyruvic acid and 3-indoleacetic acid are highly linked to ET-1, SP, and MTL levels. Conclusively, BENs, mitigating constipation by regulating gut microbiota and microbial tryptophan metabolism, showed high potential to be developed as alternative regimens for constipation.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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