Temperature-Dependent Relationship of Autophagy and Apoptotic Signaling During Cold-Water Immersion in Young and Older Males
King KE, McCormick JJ, Kenny GP
Advanced biology · 4 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Natural Sciences and Engineering Research Council of Canada
- Government
- National Sciences and Engineering Research Council
- Grants
- Natural Sciences and Engineering Research Council of Canada (RGPIN 2020); Natural Sciences and Engineering Research Council of Canada (rgpin-2020-03891); Natural Sciences and Engineering Research Council of Canada (03891)
Based on 2 listed funder(s).
Publication
- Published
- 2023-12-27 · Adv Biol (Weinh) · vol. 8 · issue 3 · p. e2300560
- Publisher
- Wiley
- Cited
- 10 citations · more than 85% of similar papers · 1.7× the field average
- References
- 45 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Autophagy in Disease and Therapy · Hydrogen's biological and therapeutic effects · Energy and Environment Impacts
- Keywords
- Autophagy, Apoptosis, Cold-shock domain, Peripheral blood mononuclear cell, Cell biology, In vivo, Inflammation, Heat shock protein, In vitro, Biology, Chemistry, Medicine, Immunology, Biochemistry
- MeSH
- leukocytes, mononuclear, humans, water, sirolimus, immersion, temperature, apoptosis, aged, autophagy, male
3 authors
From CA
- Kelli E. King · correspondingUniversity of Ottawa
- James J. McCormickUniversity of Ottawa
- Glen Patrick KennyUniversity of Ottawa; Ottawa Hospital; Ottawa Hospital Research Institute
Abstract
Autophagy is a crucial cytoprotective mechanism preventing the accumulation of cellular damage, especially during external stimuli such as cold exposure. Older adults poorly tolerate cold exposure and age-related impairments in autophagy may contribute to the associated reductions in cold tolerance. The purpose of this investigation is to evaluate the effect of different intensities of in vivo cold-water immersion and in vitro cold exposure on autophagic and apoptotic signaling in young and older males. Peripheral blood mononuclear cells (PBMCs) are isolated at baseline, end-cold exposure, and after 3 h of thermoneutral recovery. Additionally, PBMCs are treated with rapamycin and bafilomycin prior to in vitro cold exposure equivalent to in vivo core temperatures (35-37 °C). Proteins associated with autophagy, apoptosis, the heat shock response, and inflammation are analyzed via Western blotting. Moderate cold stress (0.5 °C decrease in core temperature) increased autophagic and heat shock protein activity while high cold stress (1.0 °C decrease in core temperature) augmented apoptosis in young males. In older males, minimal autophagic activation during both cold-water exposures are associated with increased apoptotic and inflammatory proteins. Although in vitro cold exposure confirmed age-related dysfunction in autophagy, rapamycin-induced stimulation of autophagic proteins underlie the potential to reverse age-related vulnerability to cold exposure.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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