Dietary cholesterol increases body levels of oral administered vitamin D<sub>3</sub> in mice
Kühn J, Schutkowski A, Rayo-Abella LM, Kiourtzidis M, Nier A, Brandsch C, Stangl GI
Journal of nutritional science · 3 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- Deutsche Forschungsgemeinschaft
- Grants
- Deutsche Forschungsgemeinschaft (STA 868/4-1); Deutsche Forschungsgemeinschaft (320705652)
Based on 1 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2024-01-01 · J Nutr Sci · vol. 13 · p. e50
- Publisher
- Cambridge University Press
- Cited
- 4 citations · more than 78% of similar papers · 1.1× the field average
- References
- 28 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Vitamin D Research Studies · Pharmacological Effects and Toxicity Studies · Drug Transport and Resistance Mechanisms
- Keywords
- Cholesterol, Bile acid, Internal medicine, Vitamin, Taurocholic acid, Endocrinology, Vitamin D and neurology, Chemistry, Dietary Cholesterol, Dry matter, Biology, Medicine, Animal science
- MeSH
- liver, caco-2 cells, feces, animals, mice, inbred c57bl, humans, mice, bile acids and salts, cholecalciferol, cholesterol, dietary, vitamin d, administration, oral, biological availability, dietary supplements, male
7 authors
From DE
- Julia Kühn · correspondingMartin Luther University Halle-Wittenberg
- Alexandra SchutkowskiMartin Luther University Halle-Wittenberg
- Lina-Maria Rayo-AbellaMartin Luther University Halle-Wittenberg
- Mikis KiourtzidisMartin Luther University Halle-Wittenberg
- Anika NierMartin Luther University Halle-Wittenberg
- Corinna BrandschMartin Luther University Halle-Wittenberg
Abstract
Vitamin D and cholesterol share the same intestinal transporters. Thus, it was hypothesized that dietary cholesterol adversely affects vitamin D uptake. The current studies investigated the influence of cholesterol on the availability of oral vitamin D. First, 42 wild-type mice received a diet with 25 µg/kg labelled vitamin D3 (vitamin D3-d3), supplemented with either 0% (control), 0.2%, 0.4%, 0.6%, 0.8%, 1.0% or 2.0% cholesterol for four weeks to investigate vitamin D uptake. In a second study, 10 wild-type mice received diets containing 0% (control) or 1% cholesterol over four weeks to determine cholesterol-induced changes in bile acids. Finally, we investigated the impact of cholesterol versus bile acids on vitamin D uptake in Caco-2 cells. Surprisingly, dietary cholesterol intake was associated with 40% higher serum levels of vitamin D3-d3 and 2.3-fold higher vitamin D3-d3 concentrations in the liver compared to controls. The second study showed that cholesterol intake resulted in higher concentrations of faecal bile acids (control: 3.55 ± 1.71 mg/g dry matter; 1% dietary cholesterol: 8.95 ± 3.69 mg/g dry matter; P P P In-vitro analyses revealed that taurocholic acid (P < 0.001) but not cholesterol increased the cellular uptake of vitamin D by Caco-2 cells. To conclude, dietary cholesterol seems to improve the bioavailability of oral vitamin D by stimulating the release of bile acids and increasing the hydrophobicity of bile.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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